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한외여과를 위한 다당류 층의 친수성 표면개질을 이용한 단백질 흡착제어
- Alternative Title
- Kim Yoon Hwan
- Author(s)
- 김윤환
- Alternative Author(s)
- Kim Yoon Hwan
- Advisor
- 이재의
- Department
- 일반대학원 응용생명공학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2010-02
- Language
- eng
- Alternative Abstract
- This study makes to reduce a protein adsorption to the surface and obtain a high-yield product while we carry out the separation and extraction of protein. Adsorption of Protein, the most representative material among biomolecules, occurs at surface of membrane and disturbs efficient separation and extraction. To prevent the adsorption of protein, surface of membrane was modified into hydrophilic surface. In order to obtain hydrophilic surface, we used hydrophilic polysaccharide such as Pectin, Dextran, Hyaluronic acid and chitosan which is known a effective biomolecule's adsorption preventer. In order to enhance transmittance, we make membrane having a porous structure by using electrospinning. The modified surface of membrane was observed by SEM(Scanning Electron Microscope) and AFM(Atomic Force Microscope) and concentration amounts and adsorption amounts of protein were measured by BCA(Bicinchoninic acid) assay. And ultrafiltration system was used to investigate water flow rate. And the hydrophilic group is introduced into polysaccharide layer. Through this study, the protein adsorption of surface coated by polysaccharide was affected by electrostatic property between protein and polysaccharide. And the higher hydrophilicity of surface coated by polysaccharide is, the lower adsorption ratio of protein is. Also the membrane introducing MPEG-COOH on chitosan layer have an excellent performance regarding prevention of protein adsorption. These results lead us to the conclusion that membrane of several types were prepared by introducing functional groups
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Applied Biotechnology > 3. Theses(Master)
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