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알츠하이머 모델 쥐에 우측 총경동맥 폐색을 일으켜 유발된 혼합형치매
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 주인수 | - |
| dc.contributor.author | Lee, Jin Soo | - |
| dc.date.accessioned | 2019-10-21T07:13:13Z | - |
| dc.date.available | 2019-10-21T07:13:13Z | - |
| dc.date.issued | 2010-02 | - |
| dc.identifier.other | 10574 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/17403 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :의학과,2010. 2 | - |
| dc.description.tableofcontents | ABSTRACT i TABLE OF CONTENTS iii LIST OF TABLES vi LIST OF FIGURES vii I. INTRODUCTION 1 A. Vascular factors in patients with Alzheimer disease 1 B. Induction of chronic cerebral hypoperfusion in Alzheimer disease models 2 C. Tg2576, an Alzheimer disease mouse model 3 D. Right common carotid artery occlusion, a chronic cerebral hypoperfusion mouse model 4 E. Purpose of study 5 II. MATERIALS AND METHODS 6 A. Materials 6 1. Animals 6 2. Reagents 6 3. Antibodies 7 B. Methods 8 1. Animal preparation 8 2. Anesthetic methods 8 3. Surgical procedures of rCCAO 8 4. Cerebral blood flow assessment 9 5. Positron emission tomography to evaluate metabolic status 10 6. Tissue preparation for histological studies 10 7. Behavioral assessment 11 8. Histological studies 14 9. Experimental sets and grouping 17 10. Statistical analysis 19 III. RESULTS 20 A. Establishment of a mild ischemic condition in mice 20 B. Replication study for a vascular cognitive impairment model induced by rCCAO 24 1. A lower recognition of novel object in rCCAO-operated mice 24 2. Occasional apoptotic cell death observed 7 days after rCCAO 26 3. Hypometabolism in FDG microPET for an rCCAO-operated mouse 30 C. Tg 2576 treated with chronic mild ischemia 32 1. Consequences of surgery 32 2. Behavioral tests 35 3. Histopathological tests 40 IV. DISCUSSION 46 A. Cognitive deterioration induced by rCCAO in Tg2576 mice 46 B. Vascular cognitive impairment induced by rCCAO 50 C. A review of vascular cognitive impairment 53 1. Subtypes of VCI 53 2. Neuropsychological features of VCI 55 3. Frontal-subcortical circuit 56 V. CONCLUSION 58 REFERENCES 59 국문요약 70 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 알츠하이머 모델 쥐에 우측 총경동맥 폐색을 일으켜 유발된 혼합형치매 | - |
| dc.title.alternative | Mixed Dementia Induced by Right Common Carotid Artery Occlusion in an Alzheimer Mouse Model | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Jin Soo Lee | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2010. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 568395 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010574 | - |
| dc.subject.keyword | Alzheimer disease | - |
| dc.subject.keyword | mixed dementia | - |
| dc.subject.keyword | vascular cognitive impairment | - |
| dc.subject.keyword | common carotid artery occlusion | - |
| dc.subject.keyword | amyloid plaque | - |
| dc.subject.keyword | 알츠하이머 | - |
| dc.subject.keyword | 우측 총경동맥 폐색 | - |
| dc.subject.keyword | 혼합형치매 | - |
| dc.description.alternativeAbstract | Although vascular risk factors are associated with Alzheimer disease (AD), the effect of chronic cerebral hypoperfusion (CCH) on the progression of AD has not been widely evaluated. We aimed to evaluate whether CCH would aggravate the behavioral changes and plaque formation in an AD transgenic (Tg) mouse model. Female Tg2576 (Tg+) mice (an AD model) and their littermates (Tg-) were subjected to permanent right common carotid artery occlusion (rCCAO). The Morris water maze and object recognition tests were performed 6-8 weeks after the operation; the mice were then sacrificed. We evaluated cell death by histological studies and quantified the amount of amyloid plaques. An escape performance in the Morris water maze test was significantly impaired in Tg+ mice with or without rCCAO compared with that in Tg- mice, but this impairment was not different between Tg+ rCCAO- and sham-operated mice. Learning curve which was preserved in Tg+ sham-operated mice was impaired in Tg+ rCCAO-operated mice. On object recognition testing, Tg+ and Tg- mice with rCCAO showed a significant reduction in their discrimination ability compared with those without rCCAO but there was no difference in the discrimination ability between Tg+ and Tg- mice. On the histological examinations, there was no evident cellular death in the brains of experimental mice. The burden of amyloid plaques was not different between Tg+ rCCAO- and sham-operated mice. In conclusion, Tg+ rCCAO-operated mice had both cognitive deficits on spatial memory and non-spatial working memory induced in AD and VCI models, respectively. Moreover, an impairment of learning curve was synergistically induced from both pathomechanisms. | - |
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