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polyphenols이 염증억제에 미치는 효과
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 남석현 | - |
| dc.contributor.author | 윤상식 | - |
| dc.date.accessioned | 2019-10-21T07:11:55Z | - |
| dc.date.available | 2019-10-21T07:11:55Z | - |
| dc.date.issued | 2009-08 | - |
| dc.identifier.other | 10161 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/17152 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :생명과학과,2009. 8 | - |
| dc.description.tableofcontents | 목 차 국문요약 ............................................................................................................................... ⅰ 감사의글 ................................................................................................................................ ⅱ 목차 ........................................................................................................................................ ⅳ List of figures ...................................................................................................................... ⅶ List of tables ........................................................................................................................ ⅸ I. 서론 ..................................................................................................................................... 1 II. 실험 재료 및 방법 ........................................................................................................ 6 1. 시약 ..................................................................................................................................... 6 2. 세포의 배양 .......................................................................................................................... 6 3. 세포독성의 측정 .................................................................................................................. 6 4. β-hexosaminidase 활성측정 ........................................................................................... 7 5. Histamine 측정 .................................................................................................................... 8 6. Rat 복강비만세포의 분리 ................................................................................................... 8 7. RT-PCR 수행 ........................................................................................................................ 9 8. ELISA를 이용한 Cytokine 측정 ................................................................................... 10 9. DPPH를 사용한 전자공여능측정 .................................................................................. 11 10. LPS와 GalN을 이용한 급성패혈증 유발 ................................................................... 11 11. 혈청의 분리 ...................................................................................................................... 12 12. 혈청에서의 GOT, GPT 측정 ........................................................................................ 12 13. 간 조직의 염색 ................................................................................................................ 12 14. 통계분석 ............................................................................................................................. 13 III. 실험 결과 ............................................................................................................................ 14 1. RBL-2H3 세포주의 염증물질 탈과립에 대한 폴리페놀의 억제활성 ............................. 14 2. 폴리페놀의 전자공여능 측정 ........................................................................................... 17 3. b-hexosaminidase 억제활성과 전자공여능과의 관계 .................................................... 20 4. 호염구세포주와 복강비만세포주의 탈과립 억제효능 검정 ....................................... 22 4-1. Ionophore A23187을 이용한 RBL-2H3 세포주의 b-hexosaminidase 억제활성 .......................................................................................................................................... 22 4-2. Ionophore A23187을 이용한 RBL-2H3 세포주의 histamine 억제활성........... 25 4-3. Ionophore A23187과 PMA를 이용한 RPMC의 b-hexosaminidase 억제활성 .......................................................................................................................................... 28 4-4. Ionophore A23187과 PMA를 이용한 RPMC의 histamine 억제활성 .......... 31 4-5. DNP-IgE를 이용한 RBL-2H3 세포주의 b-hexosaminidase 억제활성 ............... 34 5. 호염구세포주의 염증성 cytokine의 분비에 미치는 폴리페놀의 효과검정 .......... 36 5-1. RT-PCR을 이용한 폴리페놀의 염증성 cytokine 분비억제효과 검정 ............. 36 5-2. ELISA를 이용한 폴리페놀의 염증성 cytokine 분비억제효과 검정 ................ 38 6. 급성패혈증 마우스모델을 이용한 폴리페놀의 억제효과 검정 ................................ 40 6-1. LPS와 GalN으로 인한 급성패혈증 in vivo 모델에서의 폴리페놀 효과 검정 ........................................................................................................................................ 40 6-2. 급성패혈증에 의한 생존실험에서의 폴리페놀 효과 .......................................... 45 IV. 고찰 및 결론 ....................................................................................................................... 47 V. 결론....................................................................................................................................... 55 VI. 참고문헌 ............................................................................................................................. 56 Abstract ..................................................................................................................................... 60 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | polyphenols이 염증억제에 미치는 효과 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 생명과학과 | - |
| dc.date.awarded | 2009. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 567981 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010161 | - |
| dc.subject.keyword | polyphenols | - |
| dc.subject.keyword | 염증억제 | - |
| dc.subject.keyword | 효과 | - |
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- Graduate School of Ajou University > Department of Bioscience > 3. Theses(Master)
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