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Gangliosides 자극에 의한 신경소교세포의 형태학적 변화에서의 tubulin의 역할에 대한 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 주 일 로 | - |
| dc.contributor.author | 박지영 | - |
| dc.date.accessioned | 2019-10-21T06:47:38Z | - |
| dc.date.available | 2019-10-21T06:47:38Z | - |
| dc.date.issued | 2006-02 | - |
| dc.identifier.other | 1228 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/16779 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의학과,2006. 2 | - |
| dc.description.tableofcontents | ABSTRACT i TABLE OF CONTENTS ii LIST OF FIGURES iv ABBREVIATION v I. INTRODUCTION 1 A. Brain inflammation 1 B. Gangliosides 2 C. Cytoskeleton 3 D. Aims of study 4 II. MATERIALS AND METHODS 6 A. Reagents 6 B. Cell culture 6 C. Immunostaining and confocal microscopy 7 D. Phase contrast microscopy 7 E. Western Blot Analysis 7 F. Enzyme-linked immunosorbent assay (ELISA) 8 G. Data analysis 8 III. RESULTS 9 A. Gangliosides induce a unique microglial morphology with branching that is different from those induced by LPS or IFN-γ. 9 B. Gmix-induced morphology is not blocked by actin depolymerization. 13 C. Gmix-induced morphology is blocked by microtubule disruption. 19 D. LPS-induced morphology is blocked by inhibition actin filaments and microtubule. 24 E. Inhibition of actin filament or microtubules are suppressed cellular functions in Gmix-stimulated microglia. 29 IV. DISCUSSION 32 V. CONCLUSION 35 REFERENCES 36 국문요약 41 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Gangliosides 자극에 의한 신경소교세포의 형태학적 변화에서의 tubulin의 역할에 대한 연구 | - |
| dc.title.alternative | Park Ji-young | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Park Ji-young | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2006. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 565077 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001228 | - |
| dc.description.alternativeAbstract | In addition to functional changes, activated microglia undergo morphological changes from a resting, ramified morphology to an activated, amoeboid shape. Gangliosides, despite their role as microglial activators, induce microglial ramifications. To identify the cytoskeletal components involved in microglial ramification, we stimulated cells with gangliosides in the absence or presence of cytoskeletal inhibitors and examined the resultant morphological changes. While LPS-induced ameboid shapes were blocked by all inhibitors, actin inhitibors and microtubule inhibitor, Ganglioside-induced ramifications were blocked by either paclitaxel or nocodazole, inhibitors of microtubule, but not by cytochalasin B or latrunculin B, which are inhibitors of actin polymerization. In contrast to these morphological changes, iNOS expression was suppressed by both inhibitors of actin filament and inhibitors of microtubule. These results indicate that ganglioside-induced ramification is more dependent on tubulin than actin and that both of these cytoskeletal components are involved in iNOS expression. | - |
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- Graduate School of Ajou University > Department of Medicine > 3. Theses(Master)
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