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미토콘드리아의 pH를 검출하기에 적합한 이광자 형광 프로브의 합성
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김환명 | - |
| dc.contributor.author | Xu Lei | - |
| dc.date.accessioned | 2018-11-08T08:18:18Z | - |
| dc.date.available | 2018-11-08T08:18:18Z | - |
| dc.date.issued | 2015-08 | - |
| dc.identifier.other | 20201 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/12641 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :에너지시스템학과,2015. 8 | - |
| dc.description.tableofcontents | Ⅰ. General Introduction 1 1. Introduction of Two-Photon Microscopy 1 2. Introduction of Intramolecular Charge Transfer (ICT) 2 3. Introduction of mitochondrial pH 3 4. Design of Two-Photon Probes for Mitochondrial pH 4 Ⅱ. Synthesis of CMP-1 and CMP-2 6 1. Synthetic route of CMP-1 6 2. Synthetic route of CMP-2 10 Ⅲ. Photophysical Data 16 1. Spectroscopic Measurements 16 2. Water Solubility 16 3. Fluorescence pH Titrations and measurement of pKa vlues 19 4. Measurement of Two-Photon Absorption (TPA) Cross Section (δ) 21 IV. Two-Photon Microscopy Bioimaging with CMP-1 and CMP-2 23 1. Photostability and Cytotoxicity 23 2. Co-localization experiments 24 3. Cell calibration 25 4. CCCP effect on mitochondrial pH 27 5. pH change inside the mitochondria upon nutrient deprivation 29 6. TPM images with CMP-2 in Live Brain Tissues 30 V. Conclusion 32 References 33 1H-NMR, 13C-NMR and HRMS spectrum of intermediates and CMP-1 36 1H-NMR, 13C-NMR and HRMS spectrum of intermediates and CMP-2 42 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 미토콘드리아의 pH를 검출하기에 적합한 이광자 형광 프로브의 합성 | - |
| dc.title.alternative | Synthesis of Benzochromene-Derived Two-Photon Fluorescence Probes for Mitochondrial pH | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | XU LEI | - |
| dc.contributor.department | 일반대학원 에너지시스템학과 | - |
| dc.date.awarded | 2015. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 758293 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020201 | - |
| dc.subject.keyword | TP Fluorescence Probes | - |
| dc.subject.keyword | Mitochondrial pH | - |
| dc.description.alternativeAbstract | Two-photon microscopy (TPM) is a fluorescence imaging technique which provides greater tissue penetration, limits the excitation volume, and induces less photobleaching, phototoxicity and photodamage. Mitochondria play a crucial role in cell metabolic processes such as energy production, cell signaling, Ca2+ regulation, production in reactive oxygen spec ies and triggering of cell death.The mitochondrial function depends on its pH. Mitochondrial pH plays a significant role in driving force for the mitochondrial cation homeostasis, production of ATP and neurotransmission an insulin secretion. Here, we report two mitochondria-targetable two-photon fluorescent pH sensitive probes (CMP-1 and CMP-2) allowing for a quantitative measurement of mitochondrial pH in living cells and tissues. In the probe architecture, we assimilated the donor OH group at the C-8 position of the benzochromene scaffold which enhance the dipole moment and offer larger intramolecular charge transfer (ICT) process. Benzochromene scaffolds of CMP-1 and CMP-2 are different. CMP-1 is bent-π-shaped and CMP-2 is linear-π-shaped. Because conjugation of CMP-2 is larger than CMP-1, the ICT process in CMP-2 would enhance and the pH monitoring with CMP-2 would be occurred in red region which is ruled out the autofluorescence and helpful for enhanced the spectral separation from cellular absorption in biological trial. | - |
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- Graduate School of Ajou University > Department of Energy Systems > 3. Theses(Master)
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