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세포전달체와 초음파를 이용한 디스크 재생
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 민병현 | - |
| dc.contributor.author | Choi, Jae Won | - |
| dc.date.accessioned | 2018-11-08T08:06:57Z | - |
| dc.date.available | 2018-11-08T08:06:57Z | - |
| dc.date.issued | 2014-08 | - |
| dc.identifier.other | 17500 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/10760 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :분자과학기술학과,2014. 8 | - |
| dc.description.tableofcontents | TABLE OF CONTENTSi LIST OF FIGURES vi LIST OF TABLES vii ABBREVIATIONS viii ABSTRACTxi Chapter I 1 1. Intervertebral Disc (IVD) 2 1.1 Anatomy and development 2 1.2 Development 6 1.3 Cell population 8 1.4 Biochemical composition 11 1.5 Properties and function 17 1.6 Intervertebral disc degeneration and diseases 20 2. Tissue engineering for IVD regeneration 23 2.1 Tissue engineering 23 2.2 Cell source and strategy for IVD regeneration 24 2.2.1 Nucleus pulposus cells 25 2.2.2 Mesenchymal stem cells 26 2.3 Cell carriers for IVD regeneration 27 2.3.1 Fibrin and fibrinogen 28 2.3.2 Hyaluronic acid 29 2.4 Cell signal inducer for IVD regeneration 30 2.4.1 LIUS (low-intensity ultrasound) 30 3. Aim and title of this study 32 CHAPTER II Disc regeneration using autologous MSCs and fibrin/hyaluronic acid composite 33 1. Introduction 34 2. Materials and Methods 37 2.1 Isolation and culture of autologous mesenchymal stem cells 37 2.2 Preparation of fibrin and hyaluronic acid (Fi/HA) composite gel 37 2.3 Study design 38 2.4 Preparation of injured IVD model and materials transplantation 39 2.5 Magnetic resonance imaging 41 2.6 Macroscopic grading of IVD 42 2.7 Histological analysis 44 2.8 Statistical analysis 46 3. Result 47 3.1 Grades of disc degeneration by MRI 47 3.2 Gross evaluation 49 3.3 Histologic examination 53 4. Discussion 56 CHAPTER III Disc regeneration using Low-intensity ultrasound (LIUS) 60 1. Introduction 61 2. Materials and Methods 63 2.1 Study design 63 2.2 Preparation of injured intervertebral disc model 63 2.3 Treatment of Low-intensity ultrasound (LIUS) 66 2.4 Histological analysis 66 3. Results 67 3.1 Gross finding of intervertebral injury 67 3.2 Proteoglycan evaluation by safranin-O 69 3.3 Evaluation of collagen structure and deposition 69 4. Discussion 75 Chapter IV 78 REFERENCE 79 국문요약 88 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 세포전달체와 초음파를 이용한 디스크 재생 | - |
| dc.title.alternative | Jae Won Choi | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Jae Won Choi | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2014. 8 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | 652670 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017500 | - |
| dc.subject.keyword | regeneration | - |
| dc.subject.keyword | cell carrier | - |
| dc.subject.keyword | fibrin | - |
| dc.subject.keyword | hyaluronic acid | - |
| dc.subject.keyword | injection | - |
| dc.subject.keyword | autologous cell transplantation | - |
| dc.subject.keyword | mesenchymal stem cells | - |
| dc.subject.keyword | intervertebral disc | - |
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- Graduate School of Ajou University > Department of Molecular Science and Technology > 4. Theses(Ph.D)
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